Your thawing protocol may fluctuate dependant upon your cell kind, and it is suggested to refer to the protocol supplied by your seller. Here is a standard protocol for freezing frozen Principal cell solutions using a 37°C drinking water bathtub.

This will permit the cells to freeze at somewhere around -one°C/moment, that's ideal for freezing most cell kinds. You can also decide to make use of a controlled charge freezer and store the vials directly at -eighty°C.

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Take a look at this site to know the fundamentals of cell cryopreservation and to locate best tactics for freezing down your cells.

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